Efficacy and safety of the removal of intestinal endotoxin in the blood by affinity adsorption materials / 中国实验动物学报

ABSTRACT

Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intes-tinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perfo-ration of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment,we moni-tored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was(0.49 ± 0.22),(0.034 ± 0.00)Eu/mL after hemoperfusion for 1 and 2 hours,compared with that before the beginning of perfusion(7.25 ± 1.18)Eu/mL,showing a significant difference(P <0.05). After treatment for 2 hours,the average clearance rate was 99.52%. White blood cells,red blood cells,hemoglo-bin,platelets,total protein,albumin,globulin,alanine aminotransferase,aspartate aminotransferase,urea and creatinine index were significantly decreased after perfusion(P < 0.05). The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively re-move endotoxin in the blood of experimental dogs and has good blood compatibility.