Effect of E2F3 gene interfering on the invasion and migration of prostate cancer cells / 中国比较医学杂志

ABSTRACT

Objective To investigate the effect of interfering with E2F3 gene expression on the invasion and migration of prostate cancer cells and its mechanism. Methods The expression of E2F3 gene in human prostate cancer Du145 cells was knocked down by siRNA. The cells were divided into three groups control group, Du145 NC group (siRNA-NC)and Du145-siRNA group(siRNA-E2F3). Cell migration and invasion were detected by Transwell invasion and wound healing assay. The expressions of E2F3, E-cadherin and MMP-9 proteins were detected by western blotting. Results After transfection, the expression of E2F3 protein in the Du145-siRNA group was significantly lower than the control group(P< 0.01). The number of invasive cells and wound healing rate of Du145 cells in the Du145-siRNA group were significantly lower than the control group(P < 0.01). Furthermore, the protein expression of E-cadherin was significantly increased(P< 0.01)while MMP-9 decreased(P< 0.01)in the Du145-siRNA group. Conclusions E2F3 silencing can inhibit the invasion and migration ability of prostate cancer Du145 cells, and this might be accomplished by regulating E-cadherin and MMP-9 protein.