Effects of galectins on the apoptosis in HIV-1-infected macrophages / 中国比较医学杂志

ABSTRACT

Objective To investigate the effects of galectin-2, galectin-4, galectin-7, galectin-8, and galectin-9 on the apoptosis in HIV-1-infected macrophages and to provide the theoretical and application basis for elimination of HIV-1-infected cellular reservoirs. Methods Firstly, apoptosis of human monocytic cell line THP-1 cells was induced by different concentrations of galectins to determine the suitable concentration of different galetcins. Secondly, monocytes (THP-1) were stimulated to differentiate into macrophages (THP-1-Mφ) with phorbol myristate acetate (PMA), and then macrophages were prepared and infected with HIV-1. Finally, HIV-1-infected and uninfected macrophages were respectively treated with the suitable concentrations of galectin-2, galectin-4, galectin-7, galectin-8, galectin-9 and then the apoptosis in these macrophages was detected. Results The cell death rate of macrophages without treatment was 4. 39 ± 0. 74% . The cell death rates of macrophages induced by 5 μmol/L galectin-2, 5 μmol/L galectin-4, 7. 5 μmol/L galectin-7, 3 μmol/L galectin-8 and 1 μmol/L galectin-9 were 4. 78 ± 0. 41% , 7. 21 ± 1. 46% , 3. 78 ± 1. 03% , 5. 88 ± 2. 08% , 8. 10 ± 4. 13% , respectively, with no statistically significant defferences among the groups (P＞ 0. 05). The cell death rate of HIV-1-infected macrophages without treatment was 12. 69 ± 1. 16% , and that of HIV-1-infected macrophages induced by 5 μmol/L galectin-2, 5 μmol/L galectin-4, 7. 5 μmol/L galectin-7, 3 μmol/L galectin-8 and 1 μmol/L galectin-9 were 11. 69 ± 0. 90% , 17. 45 ± 1. 30% , 32. 01 ± 1. 30% , 15. 77 ± 1. 21% and 19. 27 ± 2. 13% , respectively. There were significant differences between the control group and galectin-7-treated group (P ＜ 0. 001 ). Conclusions Galectin-7-induces extensive apoptosis in HIV-1-infected macrophages but not in uninfected macrophages.