Intervention impact of Wogonin on type 1 diabetic mice and its influence on p62dok expression in liver / 中国糖尿病杂志

ABSTRACT

Objective To explore the Intervention impact of Wogonin on type 1 diabetic mice and its influence on p62dok expression in liver.Methods Fifty SPF level male C57BL/6 mice were randomly divided into 5 groupscontrol group (Con,n =10),diabetes group (STZ group,n =40).Diabetic model was successfully made by STZ.After that,7 were chosen as model group,and then STZ group was subdivided into three subgroups according to different doses of Wogonin5,10,and 20 mg/kg subgroup (n =10/subgroup).Fasting blood glucose levels were measured in all the subjects.And intraperitoneal injection of glucose tolerance test (IPGTT) was used to evaluate he glucose tolerance.Serum insulin level was tested by ELISA kits.Dok1,Dok2 and AKT protein expression level in liver tissue were measured by western blot.Results Wogonin could significantly reduce FPG in STZ induced type 1 diabetic mice [(12.55 ±1.31) vs (7.24 ±0.49) vs (6.22 ± 0.69) mmol/L,P ＜ 0.05].Wogonin could improve the glucose tolerance,and restore the serum insulin levels to normal in type 1 diabetic mice induced by STZ.Wogonin could increase Dok1[(0.29±0.09) vs (0.68±0.14) vs (0.79±0.13)] and Dok2[(0.32±0.08)vs (0.61±0.07) vs (0.84±0.12)] expression levels in the liver of type 1 diabetic mice induced by STZ (P＜0.05).Serum insulin-level was significantluy higher in10 mg/kg Wogonin intervention group and 20mg/kg Wogonin intervention group than in model group (P＜0.05),indicating that serum insulin level increased after Wogonin intervention.Conclusion Wogonin could significantly alleviate hyperglycemia and hyperinsulinemia in STZ induced type 1 diabetic mice,and could stimulate the expression of Dok1 and Dok2 in the liver tissue of diabetic mice.