Effects of insulin-like growth factor binding protein 7 gene on the biological characteristics of colorectal cancer / 中国现代普通外科进展

ABSTRACT

Objective:To investigate the effects of insulin-like growth factor binding protein 7 gene on the biological characteristics of colorectal cancer.methods:human colorectal cancer SW480 cells were cultured.According to the different transfectants,the cells were divided into IGFBP7 plasmid group,blank plasmid group and control group.The expression of IGFBP7 gene was detected by RT-PCR.MTT assay was used to detect cell proliferation.Flow cytometry was used to detect the apoptotic rate.Transwell chamber was used to detect cell migration and cell invasion.Results:The relative expression levels of IGFBP7 mRNA in IGFBP7 plasmid group was higher than which in the blank plasmid group and the control group,the difference was statistically significant (P＜0.05).Compared with the blank plasmid group and the control group,the A values at 24h,48h,72h and 96h in the IGFBP7 plasmid group were decreased,the differences were statistically significant(P＜0.05).Compared with the blank plasmid group and the control group,the apoptosis rate in the IGFBP7 plasmid group was increased,the difference was statistically significant (P＜0.05).The number of migrating cells and the number of invasive cells in IGFBP7 plasmid group were lower than those in the blank plasmid group and the control group,the differences were statistically significant (P＜0.05).Conclusion:Overexpression of IGFBP7 gene could inhibit proliferation of colorectal cancer cells,accelerate cell apoptosis,reduce cell migration and cell invasion.