Correlation Study Between Peripheral Blood Levels of Let-7e, miRNA-206, miRNA-605, miRNA-1236 and Essential Hypertension Occurrence / 中国循环杂志

ABSTRACT

Objective: To explore the relationship between peripheral blood levels of Let-7e, miRNA-206, miRNA-605, miRNA-1236 and essential hypertension (EH) occurrence. Methods: Our research included in 2 groups HE group, n=169 patients treated in our hospital from 2014-07 to 2016-12 and Control group, n=143 healthy subjects from physical examination at the same period. Blood pressure was measured by sphygmomanometer, the expression levels of peripheral Let-7e, miRNA-206, miRNA-605 and miRNA-1236 were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Relationship between miRNA levels and HE occurrence was studied by multivariate Logistic regression analysis. Results: Compared with Control group, EH group showed increased expressions of Let-7e and miRNA-605 (1.42±0.15) vs (1.00±0.21) and (2.14±0.22) vs (1.00±0.11), both P<0.05; decreased expressions of miRNA-206 and miRNA-1236 (0.69±0.12) vs (1.00±0.18) and (0.46±0.17) vs (1.00±0.31), both P<0.05. Correlation analysis presented that peripheral level of Let-7e was positively related to systolic blood pressure (r=0.562, P<0.05); miRNA-206 was negatively to systolic blood pressure (r=-0.582,P<0.05)and positively related to diastolic blood pressure(r=0.507,P<0.05);miRNA-605 was positively related to systolic blood pressure (r=0.625, P<0.05); miRNA-1236 was negatively related to diastolic blood pressure (r=-0.598, P<0.05) and positively related to BMI (r=0.690, P<0.05). Multivariate Logistic regression analysis indicated that elevated BMI could increase the risk of EH occurrence (OR=2.6412, 95%CI 1.7933-3.9941, P<0.01), elevated expression of Let-7e and miRNA-605 could increase the risk of EH occurrence (OR=1.6505 , 95%CI 1.5378-1.7632, P<0.05) and (OR=1.9893, 95%CI 1.5983-2.4203, P<0.01); reduced expression of miRNA-206 and miRNA-1236 could also increase the risk of EH occurrence (OR=0.7164, 95%CI 0.5546-0.8782, P<0.01)and(OR=0.4218,95%CI 0.2876-0.5614,P<0.01). Conclusion: Peripheral blood levels of Let-7e, miRNA-206, miRNA-605 and miRNA-1236 were closely related to EH occurrence.