Enzymatic synthesis of mogroside ⅢE / 中国药科大学学报
Journal of China Pharmaceutical University
; (6): 354-359, 2018.
Article
in Zh
| WPRIM
| ID: wpr-704346
Responsible library:
WPRO
ABSTRACT
The aim was to develop the simple preparation method of mogroside ⅢE,and to lay the foundation for the development of the mogroside sweeteners. In the present study,the glycosidase CPU-GH17,which can regio-selectively biosynthesize mogroside ⅢE from mogroside V,was screened from the established library of glycosi-dases. Then,the soluble expression condition of CPU-GH17 in E. coli was exploited by investigating isopropyl β-D-thiogalactoside (IPTG)concentration,culture temperature and induction time,and 0. 4 mmol/L IPTG,15 °C and 12 h was used as optimal condition. The result showed that mogroside V could be completely converted into mogroside ⅢE under the conditions of pH 6. 0,45 °C,3 U/mL enzyme loading,5 mg/mL substrate concentration for 20 h. In conclusion,a biosynthetic system for the regio-selective preparation of mogroside ⅢE by recombinant CPU-GH17 was successfully established and verified at a preparative scale.
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WPRIM
Language:
Zh
Journal:
Journal of China Pharmaceutical University
Year:
2018
Type:
Article