Determination of the Concentration of Gabapentin in Human Plasma by LC-MS/MS / 中国药房
China Pharmacy
;
(12): 4496-4499, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-704444
ABSTRACT
OBJECTIVE:
To establish a method for the concentration determination of gabapentin (GBP) in human plasma.METHODS:
After precipitated by methanol,using sulfamethoxazole as intemal standard,LC-MS/MS method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of water (containing 0.05% formic acid)-methanol using a gradient elution program at the flow rate of 1 mL/min.The column temperature was 30 ℃,and sample size was 20 μL.The ESI was equipped and quantitative analysis was operated in positive ion and MRM mode.The mass transition ion-pairs were followed as m/z 172.0→154.1(GBP) and m/z 279.0→124.0 (internal standard).RESULTS:
The linear range of GBP was 13.4-10 720.4 ng/mL (r=0.992 3,n=5).The limit of quantitation was 13.4 ng/mL,and the minimum detection limit was 4.0 ng/mL.RSDs of inter-day and intra-day were all lower than 10%.Relative errors ranged-4.93%-5.10%.The recoveries ranged 86.2%-90.3% (RSD<5%,n=6),and matrix effects ranged 87.6%-92.1%.The plasma concentration of GBP in 10 epileptic patients ranged 2 075.19-4 078.87 ng/mL (n=20).CONCLUSIONS:
The method is proved to be sensitive,specific,practical and suitable for plasma concentration monitoring and pharmacokinetic study of GBP in epileptic patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2017
Type:
Article
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