Rapid Determination of Ginsenoside Rg1, Re, Rb1 in Panax quinquefolius Pieces by NIRS Combined with PLS Algorithm / 中国药房

ABSTRACT

OBJECTIVE:To establish the method for rapid determination of ginsenoside Rg1,Re,Rb1 in Panax quinquefolius crude slices.METHODS:HPLC method was adopted to determine the total contents of ginsenoside Rg1,Re,Rb1 (as reference value).NIRS combined PLS algorithm were adopted to establish total quantitative correction model of ginsenoside Rg1,Re,Rb1.According to the reference,62 samples were collected.The spectrum was pretreated with multivariate scattering correction method combined with first order derivative method.The optimal ranges of wave band for ginsenoside Rg1,Re,Rb1 were 7 664.23-5 236.05 cm-1.RESULTS:Methodology validation for total content determination of ginsenoside Rg1,Re,Rb1 was in line with the requirements.For total quantitative correction model of ginsenoside Rg1,Re,Rb1,related correction set coefficient was 0.991 03,corrected mean square deviation 0.010 26.CONCLUSIONS:The method is rapid,accurate,simple and free of contamination.It can be used for rapid determination of ginsenoside Rg1,Re,Rb1 in P quinquefolius crude slices.