Study on DNA Barcode Identification Method of Saiga tatarica and Its Adulterants Based on CO Ⅰ Gene / 中国药房

ABSTRACT

OBJECTIVE:To investigate the feasibility of molecule identication of Saiga tatarica and its adulterants by using cytochrome C oxidase subunit Ⅰ (CO Ⅰ) gene.METHODS:A total of 7 horns and incomplete horns were collected from 4 areas.The extraction effect of DNA from bone plug and stratum corneum were investigated;PCR technology was used to amplify CO Ⅰ gene of samples using universal primer LCO Ⅰ 490,HCO2198;after gel electrophoresis,purification (750 bp strip) and sequencing,using CO Ⅰ gene as barcode alignment sequence,online comparison was conducted by using Blast software of NCBI database to determine specific species.RESULTS:The extraction of DNA from stratum corneum was better (DNA concentration was 15.7-22.6 ng/μL,the absorbance of 260 nm/280 nm was 1.73-4.72).Online comparison showed that the similarity of CO Ⅰ gene in all sampies reached 99％,mainly from the horns of saiga antelope,Tibetan antelope,gazelle,sheep and goats.CONCLUSIONS:DNA barcode technology based on CO Ⅰ gene can be used for the identification of S.tatarica and its adulterants.The technology can provide an accurate and objective method for the identification of horn medicinal materials.