Study on Chemical Constituents of Ethanol Extract from Cirtus aurantium / 中国药房

ABSTRACT

OBJECTIVE: To study the chemical constituents of ethanol extract from Cirtus aurantium. METHODS: The ethanol extract of C. aurantium was isolated and purified by silica gel column, open ODS column, preparation liquid chromatography, HW-40F gel column and macroporous resin column. The structure of compounds was analyzed and identified according to physicochemical properties and spectral data (mass spectrum, hydrogen spectrum, carbon spectrum). RESULTS: Eight compounds were isolated from ethanol extract of C. aurantium, i. e. Rimboxo (1), Thymidine (2), Uracil (3), Cyclo-(Leu1-Ile2-Ala3-Thr4-Gly5-Thr6-Phe7) (4), Cyclo- (Leu1-Leu2-Pro3-Tyr4-Gly5-Ser6-Pro7) (5), Meranzin hydrate-β-D-glucoside (6), Umbelliferone (7), Linaloyl glucoside (8). CONCLUSIONS: Compound 1, 2, 3, 8 are isolated from Citrus L. for the first time, compound 4, 5, 6 were isolated from C. aurantium for the first time. The study lays the foundation for quality evaluation of C. aurantium.