Study on the Optimization of Water-extraction Technology of Yao Medicine Jasminum lanceolarium and the Anti-inflammatory and Analgesic Effect of Its Water Extract / 中国药房

ABSTRACT

OBJECTIVE:To optimize the water-extraction technology of Yao medicine Jasminum lanceolarium and study the anti-inflammatory and analgesic effect of its water extract. METHODS:The single factor test and orthogonal test were used to investigate the effects of extraction time,liquid-solid ratio and extraction times on water-extraction technology and optimize extraction technology using ferulic acid content and dry extract yield as index. The validation test was also conducted. With aspirin as positive control,the method of ear edema induced by xylene and paw edema induced by carrageenan in mice were used to observe the anti-inflammation effects of low-dose,medium-dose and high-dose(3,6,12 g/kg,by crude drug)of J. lanceolarium water extract;the analgesic effect of those was investigated by hot-plate induced pain response test and mice writhing response test. The contents of TNF-α and IL-1 β were determined by ELISA after carrageenan induced inflammation in mice. The contents of PGE2in serum and inflammation tissue were detected by UV spectrophotometry.RESULTS:The optimal extraction technology was as follows as extraction time of 120 min,liquid-solid ratio of 161(mL/g),extracting for 3 times.Compared to model group,the ear edema degree and paw edema rate of mice were raduced,writhing response times were lessened,and pain response threshold was enhanced significantly in low-dose,medium-dose and high-dose groups of J. lanceolarium water extract(P<0.05 or P<0.01). Compared to model group,the contents of TNF-α,IL-1β and PGE2in serum and the content of PGE2in inflammation tissue of mice were raduced significantly in low-dose,medium-dose and high-dose groups of J. lanceolarium water extract(P<0.05 or P<0.01). CONCLUSIONS:The optimized water-extraction technology of Yao medicine J. lanceolarium shows high efficiency,stable and feasible.Water extract shows significant anti-inflammatory and analgesic effects through reducing the contents of TNF-α,IL-1β and PGE2.