Study of Transfer Rate of Standard Decoction of Dipsacus asper Decoction Pieces / 中国药房
China Pharmacy
;
(12): 2782-2785, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-704887
ABSTRACT
OBJECTIVE:
To study transfer rate of asperosaponinⅥ in standard decoction of Dipsacus asper decoction pieces.METHODS:
The content of asperosaponin Ⅵ in Dipsacus asper decoction pieces and its standard decoction was determined by HPLC. The determination was performed on SinoChrom ODS-AP with mobile phase consisted of acetonitrile-water(30∶70,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 212 nm,and column temperature was 30℃. The sample size was 10 μL. By the ingredients content obtained,the transfer rate of asperosaponin Ⅵ was calculated during decoction piece to standard decoction.RESULTS:
The linear range of asperosaponin Ⅵ was 0.484-4.84 μ g(r=0.999 9). RSDs of precision,stability and reproducibility tests were all lower than 2%. The limits of quantification and detection were 0.3 and 0.1 μ g,respectively. The average recoveries in D. asper decoction pieces and standard decoction were 95.13% -100.22%(RSD=1.78%,n=6), 97.07%-100.08%(RSD=0.98%,n=6). RSD of durability test was lower than 1%.The transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces ranged 26.3%-49.5%.CONCLUSIONS:
The method is simple,accurate,precise, stable,reproducible and durable,and can be used for transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2018
Type:
Article
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