Simultaneous Determination of Loganic Acid and Isoscoparin in Sanwei Longdanhua Tablets by HPLC / 中国药房
China Pharmacy
;
(12): 2810-2813, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-704893
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.METHODS:
HPLC method was adopted. The determination was performed on Inertsil ODS-3 column with mobile phase consisted of methanol-0.2% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm,and column temperature was 30 ℃. The sample size was 10 μL.RESULTS:
The linear range were 0.040 08-4.008 0 μg(r=0.999 9)for loganic acid and 0.021 96-2.196 0 μg(r=0.999 9)for isoscoparin. The quantitative limits were 0.160 32 and 0.087 8 ng/mL,and detection limits were 0.080 16 and 0.043 92 ng/mL. RSDs of precision,stability and reproducibility tests were all lower than 2%. The recoveries were 103.07%-104.26%(RSD=0.52%,n=6) and 95.57%-99.61%(RSD=1.55%,n=6).CONCLUSIONS:
The method is simple,accurate and suitable for simultaneous determination of loganic acid and isoscoparin in Sanwei longdanhua tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2018
Type:
Article
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