Mechanism of Inhibition of Neuroblastoma Cell Proliferation by Elemene / 中国医科大学学报

ABSTRACT

Objective To investigate the effect of elemene and the mechanism of this effect on the proliferation of neuroblastoma.Methods We treated cultured SK-N-SH cells with elemene and used the MTT assay to determine the inhibition of cell proliferation.We used RT-qPCR and Western blotting to measure the effect of elemene on mRNA and protein expression,respectively,of repressor element-1 silencing transcription factor (REST) in SK-N-SH cells and evaluated the mRNA expression of CCND1 and CCNE1 in SK-N-SH cells by using RT-qPCR.Results Elemene significantly inhibited the proliferation of SK-N-SH cells in a dose-and time-dependent manner (P =0.001 16).Moreover,the mRNA (P =0.000 38) and protein (P =0.003 39) expression of REST were markedly suppressed by elemene.Furthermore,elemene significantly reduced the mRNA expression of CCND1 (P =0.001 91) and CCNE1 (P =0.000 15),which are related to the cell cycle.Conclusion Elemene significantly suppressed the proliferation of neuroblastoma cells through the reduction of REST,CCND1,and CCNE1.