Expression and Action Mechanism of miR-98 in Colon Cancer Tissues / 中国医科大学学报
Journal of China Medical University
;
(12): 9-12, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-704958
ABSTRACT
Objective To investigate the expression and action mechanism of miR-98 in colon cancer tissues. Methods Tumor tissues and adjacent tissues were collected from 40 patients with colorectal cancer. The expression of miR-98 in tumor tissues and adjacent tissues was detected by real-time PCR. miR-98 was overexpressed or silenced in cells,and the effects on proliferation,cell cycle,and apoptosis were analyzed using MTT,flow cytometry,and Hoechst 33258 assays. Results Real-time PCR showed that the expression of miR-98 in tumor tissues was lower than that in adjacent tissues (P = 0.022). The survival rate of patients with lower miR-98 expression was shorter than that of patients with higher miR-98 expression. The MTT assay showed that miR-98 overexpression inhibited the proliferation of HCT116 cells. Hoechst 33258 staining showed that the overexpression of miR-98 could inhibit the cell cycle and promote the apoptosis of HCT116 cells. Conclusion miR-98 can inhibit the proliferation and promote the apoptosis of colon cancer cells. The expression of miR-98 is closely related to the survival of patients with colon cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Medical University
Year:
2018
Type:
Article
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