Effects of notoginsenoside R1 on cutaneous wound healing in mice through different ways of administration / 中国药理学通报

ABSTRACT

Aim To study the effects of notoginsen-oside R1 on cutaneous wound healing in mice through different ways of administration. Methods The cuta-neous wound models were operated on the back of the ICR mice by punch. Then 30 mg·kg-1of notoginsen-oside R1 was given by intragastric administration(ig), intraperitoneal injection (ip) and transdermal delivery (tdd). Each group was injected once a day and lasted totally 14 days. Photos were taken for the cutaneous wound of each group on day 0, day 1, day 3, day 5, day 7,day 9,day 11,day 13 and day 14,respective-ly. The tissues of back skin around the wound were collected on day 1, day 3 and day 7. The growth of granulation tissue,collagen fiber formation and inflam-matory cell infiltration in cutaneous wound edge were observed by HE. ELISA was used to detect the protein expression of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), vascular cell adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1). The real-time RT-PCR was used to detect mRNA expression levels of collagen type I alpha 1 chain (COL1A1), collagen type Ⅲ alpha 1 chain (COL3A1), transforming growth factor-beta 1 (TGF-β1), platelet derived growth factor (PDGF)and vascular endothelial growth factor (VEGF) in wounds. Results Notoginsenoside R1 with these dif-ferent ways of administration was effective in promoting cutaneous wound healing in mice,and the effect of in-traperitoneal injection was more significant, as was shown in wound area ( % of Day 0 ) analysis. HE staining showed that notoginsenoside R1 could promote the growth of granulosa tissue,accelerate the formation of collagen fibers and reduce the infiltration of inflam-matory cells. ELISA results indicated that notoginsen-oside R1 effectively reduced the protein expression of various inflammatory factors in wound tissues. Real-time RT-PCR results showed that notoginsenoside R1 markedly up-regulated the mRNA levels of various growth factors, including TGF-β1, PDGF and VEGF. Conclusions Intraperitoneal injection of notoginsen-oside R1 shows potent effects on inhibiting inflamma-tion around the wound. Thus, intraperitoneal injection is the best among these different ways of notoginsen-oside R1 administration which could promote cutaneous wound healing.