Relationship between apolipoprotein A-Ⅱ and adriamycin resistance / 中国药理学通报

ABSTRACT

Aim To study the relationship between apolipoprotein A-Ⅱ (APOA-Ⅱ) and adriamycin re-sistance. Methods The drug sensitivity of cells was analyzed by IC50assay. The localizations of ADM in MCF7/W,MCF7/ADM and APOA-Ⅱ transgenic cells were observed by laser scanning confocal microscopy. RT-PCR and Western blot were applied to detect the gene and protein expression in MCF7/W and MCF7/ADM cells, respectively. The APOA-Ⅱ transgenic HEK293 cells were analyzed by IC50assay,the IC50of breat cancer cells T470 and MDA-MB231 were ana-lysed as well. Results Compared with MCF7/W cells,the resistance index of MCF7/ADM cells got 13. 0 (P<0.05). ADM localized mainly in the nuclei of MCF7/W cells,but none in the nuclei of MCF7/ADM cells. Meanwhile,the localization of ADM in APOA-Ⅱtransgenic MCF7/W cells was obviously less than that in normal parent MCF7/W cells. The gene and protein expression levels of APOA-Ⅱ in MCF7/ADM cells were both higher than in MCF7/W cells (P<0.05);the sensitivity of APOA-Ⅱtransgenic HEK293 to ADM was also reduced (P<0.05). Furthermore,the sensi-tivity of breast cancer cells T47D and MDA-MB231 to ADM was reduced as well (P<0.05). Conclusion APOA-Ⅱ is found to be positively related to ADM re-sistance,which would provide instruction for the clini-cal usage of ADM.