Paeoniflorin-6′-O-benzene sulfonate influences expression of CXCL12/CXCR4 in spleen of adjuvant-induced arthritis / 中国药理学通报

ABSTRACT

Aim To observe the expression of CXCL12 and its receptor CXCR4 in spleen of rats with adjuvant-induced arthritis (AA) and the effects of paeoniflorin-6′-O-benzene sulfonate (CP-25). Methods AA rats were induced using complete Freund's adjuvant and were randomly divided into normal group,AA group, CP-25 group (50 mg·kg-1) and methotrexate group (MTX,0.5 mg·kg-1),which were treated from d 14 to d 28. HE staining was used to assess the pathologi-cal changes of spleen. The expression of CXCL12 and CXCR4 in spleen was detected by ELISA,immunohis-tochemitry and Western blot. Results CP-25 (50 mg ·kg-1)alleviated the pathological changes of spleen and decreased the expression of CXCL12 and CXCR4 in spleen of AA rats. The pathological changes of spleen and the expression of CXCL12/CXCR4 in spleen revealed a positive correlation. Conclusions Increased expression of CXCL12 and its receptor CX-CR4 may be associated with the pathological changes of spleen in AA rats,which plays an important role in the pathogenesis of RA. CP-25 has obvious therapeutic effect on AA rats and its mechanism may be related to the inhibition of the expression of CXCL12 and CXCR4 in the spleen.