Protective effect of immunomodulating peptide(PGPIPN) derived from beta-casomorphin in bovine milk on acute alcohol-induced liver injury / 中国药理学通报

ABSTRACT

Aim To investigate the protective effect of immunomodulating peptide(PGPIPN) on the acute al-coholic liver injury in mice. Methods Kunming mice were randomly divided into control group, model group,glutataione(GSH) group, PGPIPN low dose group, PGPIPN moderate and high dose groups. The mice were treated with different doses of PGPIPN or GSH for two weeks except control group and model group. The acute alcoholic liver injury model was in-duced by gavage with 56° alcohol for three days. The indices including the activities of AST,ALT in serum, and the contents of TNF-α, MDA, SOD and GSH-Px in liver were examined. Liver histopathological changes were examined by HE staining. Results Compared with control group,the levels of serum ALT,AST and the contents of TNF-α, MDA significantly increased, while the contents of SOD and GSH-Px significantly de-creased in model group. There was hepatocyte apopto-sis and inflammatory cell infiltration in liver tissues. Compared with model group, the activities of serum ALT, AST and the contents of TNF-α, MDA were re-markably reduced in PGPIPN high dose group. The contents of SOD and GSH-Px significantly increased in PGPIPN high dose group. PGPIPN could alleviate the injury of liver. Conclusion PGPIPN has certain pro-tective effect on acute alcoholic liver injury of mice, providing a theoretical guidance.