Effect of soyasaponin on drug resistance of ovarian cancer cells / 中国药理学通报

ABSTRACT

Aim To screen the most effective soyasa-ponins for reversing the drug resistance of ovarian cancer cells, and investigate the possible mechanism. Methods Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin. Then, the most effective soyasaponin was used to detect the ex-pression of epithelial-mesenchymal transition ( EMT)-related marker proteins, including N-cadherin and E-cadherin,with Western blot and confocal microsco-py. Finally, transwell assay and wound healing assay were applied to observe effect of soyasaponin on regula-ting cancer cell migration. Results Compared with other soyasaponins,soyasaponin Ac most effectively re-versed the drug resistance of A2780/PTX cells. The expression of N-cadherin decreased while that of E-cadherin increased in A2780/PTX cells when treated with soyasaponin Ac for 48 h. The results of transwell and wound healing assay suggested that soyasaponin Ac also reduced the migration of A2780/PTX cells. Con-clusion Soyasaponin Ac can decrease the drug resist-ance via EMT pathway and weaken the migration abili-ty of ovarian cancer cells.