Determination of Paraquat in Human Serum by HPLC / 中国药师
China Pharmacist
;
(12): 1919-1921, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-705390
ABSTRACT
Objective:
To establish an HPLC method for the determination of paraquat in human serum.Methods:
The analytical column was a Kromasil C18column (200mm×4.6mm,5μm).The mobile phase was a mixture of acetonitrile and water ( containing 0.03 mol·L-1sodium heptanesulfonate and 0.24 mol·L-1phosphoric acid) (397,pH was adjusted to 2.0 by triethylamine),the detection wavelength was set at 258 nm,the column temperature was 25℃,the injection volume was 20 μl,and the flow rate was 0.8 ml·min-1.Results:
The calibration curve of paraquat was linear within the range of 0.106-10.6 mg·L-1( r =0.999 3),and the lower limit of detection was 0.065 mg·L-1. The absolute recovery of paraquat at low,medium and high concentration was more than 89.4%,and the method recovery was more than 94.4%. The intra-day RSDs were 0.12%-1.74%,and the inter-day RSDs were 0.44%-2.89%.Conclusion:
The method is simple,quick,accurate,sensitive and specific,and can be used for detecting paraquat con-centration in human serum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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