Simultaneous Determination of Three Index Components in Fufang Fuqing Lotion by Dual Wavelengths HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method with dual wavelengths for simultaneously determining chlorogenic acid , caffe-ic acid and paeoniflorin in Fufang Fuqing lotion .Methods: The HPLC method was performed on an Inertsil C 18 column ( 250 mm × 4.6 mm, 5 μm)with the column temperature at 40℃.The mobile phase was acetonitrile-0.02％ phosphoric acid (1783) with the flow rate of 1.0 ml· min-1 .The detection wavelengths were set at 323 nm and 230 nm.Results:The calibration curve of chlorogenic acid, caffeic acid and paeoniflorin showed a good linear relationship over the range of 7.50-120.00 μg· ml-1(r=0.9999), 2.50-40.00 μg· ml-1(r=0.9998) and 14.06-225.00 μg· ml-1(r=0.9997), respectively.The average recovery was 98.55％ (RSD=1.66％,n=6), 94.52％(RSD=0.98％,n=6)and 99.18％(RSD=0.65％,n=6), respectively.Conclusion:The method is simple, quick, accurate and specific , which can be used for the quality analysis and control of Fufang Fuqing lotion .