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Simultaneows Determination of Strychnine and Brucine in Shiyifang Vinum by LC-MS/MS / 中国药师
China Pharmacist ; (12): 348-350, 2018.
Article in Chinese | WPRIM | ID: wpr-705530
ABSTRACT

Objective:

To develop a determination method for strychnine and brucine in Shiyifang vinum by LC-MS/MS. Meth-odsThe samples were separated on an Agilent Zorbax SB-C18column(2.1 mm×100 mm,3.5 μm). The mobile phase was metha-nol-10 mmol·L-1ammonium formate solution (adjusting pH to 3.0 with methane acid)(25 75), and the flow rate was 0.2 ml· min-1. The eletrospray ionization( ESI) with positive source of the mass spectrometer and multiple reaction monitor( MRM) mode were used to detect strychnine(m/z 395.2→243.8;m/z 395.2→212.8)and brucine(m/z 335.2→183.9;m/z 335.2→155.9). Re-sultsThe linear range of strychnine was 0.242-7.733 μg·ml-1,and that of brucine was 0.302-9.661 μg·ml-1. The average re-covery was 100.1% (RSD=0.8%,n=6) and 99.4% (RSD=1.4%,n=6),respectively.

Conclusion:

The method is simple and accurate,which can be used to determine the contents of strychnine and brucine in Shiyifang vinum.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2018 Type: Article