Simultaneous Determination of Astragulin and Kaempferol in Bairui Granule by HPLC-MS/MS / 中国药师

ABSTRACT

Objective:To develop a quantitative HPLC-MS/MS method for the determination of astragulin and kaempferol in Bairui granule. Methods:A column of Ultimate XB-C C18(100 mm×2.1 mm,5 μm)was used. The mobile phase was composed of 0.1% formic acid-water(A) and 0.1% formic acid-acetonitrile(B) with gradient elution. The flow rate was 0.3 ml·min-1. The detection was performed with multiple teactions monitoring (MRM) using electrospray ionization (ESI). The precursor/product ion transitions were monitored at m/z 447.2→m/z 284.0 (anion mode) for astragulin and m/z 285.1→m/z 187.1 (anion mode) for kaempferol. The temperature of column was set at 40℃. Results:A good linearity was obtained with the concentration ranging from 9.86 to 1 970 ng·ml-1(r=0.999 5) for astragulin and 8.73 to 437 ng·ml-1(r=0.999 6) for kaempferol. The average recovery of the two inves-tigated compounds at low, medium and high concentrations was 99.23%, 99.65% and 99.23%, and 98.24%, 99.13% and 99.69% with the RSD of 1.84%,1.37% and 1.21%,and 1.38%,0.96% and 1.47%, respectively (n=6). Conclusion: The method is rapid,accurate and reliable,which can be used to control the quality of Bairui granule.