Determination of Residual Formaldehyde in EDTA-2Na by Headspace Gas Chromatography / 中国药师

ABSTRACT

Objective:To establish a headspace GC method for the determination of residual formaldehyde in EDTA-2Na. Meth-odsA headspace GC was used to separate the residual solvents on an Agilent DB-WAX (30 m×0.32 mm,0.5 μm) capillary col-umn with an FID detector. The carrier gas was nitrogen at the flow rate of 1.0 ml·min-1. The temperature of the injector was 230 ℃and that of the FID was 260 ℃. The programmed column temperature was set as follows maintained at 40 ℃ for 6 min, and then raised to 230 ℃ at the rate of 40 ℃·min-1and maintained for 10 min. The injection volume was 1μl with the split ratio of 21. The reference solvent and sample were filled into the containers of headspace injector and the containers were in equilibrium at 95℃ for 25 min. The amount of residual formaldehyde was calculated by an external standard method.Results:There was a good linear relationship of formaldehyde reference solvent within the concentration range of 5.10-102.00 μg·ml-1(r=0.999 7). The average recovery was 84.60%(RSD=3.82%,n=9). Conclusion: The method is simple,rapid and accurate for the determination of residual formalde-hyde in EDTA-2Na.