Determination of Sodium Glycerophosphate and Phosphate Salts in Concentrated Divitamins and Sodium Phosphate Syrup by Ion Chromatography / 中国药师

ABSTRACT

Objective:To establish ion chromatography(IC) method for determination of the content of sodium glycerophosphate and phosphate salts in concentrated divitamins and sodium phosphate syrup. Methods:An AG19 -HC (50 mm× 4 mm) guard col-umn and an IonPac AS18 -HC (250 mm × 4 mm) ion analysis column were used, potassium hydroxide solution was applied as the mobile phase with gradient elution,and a conductance detector was used with suppression conductometric detection. Results:Sodium glycerophosphate and phosphate could be separated well under the chromatographic conditions. The linear range was 1.054-84.35 μg ·ml-1(r=0.999 4)for sodium glycerophosphate and 0.316 8-25.35 μg·ml-1(r=0.999 8)for phosphate. The average recovery was 99.35% (RSD=1.16%,n=9) and 98.96%(RSD=2.61%,n=9), respectively.Conclusion: The method is simple, rapid and accurate,and can be used for the quality control of concentrated divitamins and sodium phosphate syrup.