Analysis of Volatile Components in Kaempferia Galanga Linn. from Different Habitats / 中国药师

ABSTRACT

Objective:To lay the material foundation for the research of subsequent pharmacological activities by the analysis of volatile components in Kaempferia galanga Linn. from different origins. Methods:It was the first time that headspace solid-phase microextraction and gas chromatography mass spectrometry (HS-SPME/GC-MS) technique was used to extract and analyze the volatile chemical components in Kaempferia galanga Linn. from Guangxi, Guangdong and Yunnan, and the area normalization method was used to calculate the mass fraction of each component. Results:Totally 42 chemical constituents were identified,mainly terpenoids,hydrocarbons,esters and aromatic compounds. A total of 41 chromatographic peaks were isolated from the volatile substances in Guangxi and 38 chemical constituents were identified,which accounted for 99.78% of the total of the volatile components. Totally 37 chromatographic peaks were isolated from the volatile substances in Guangdong, and 26 chemical constituents were identified, which accounted for 80.49% of the total of the volatile components. A total of 31 chromatographic peaks were isolated from the volatile compounds of Yunnan,and 24 chemical constituents were identified,which accounted for 64.72% of the total of the volatile components.Conclusion:The volatile components in Kaempferia galanga Linn. from Guangxi,Guangdong and Yunnan show little difference,and the characteristic components of the three habitats are methoxy cinnamate ethyl cinnamate, ethyl cinnamate and pentadecane, however, the relative contents of the three characteristic components from the three areas are much different,which are 45.02%,17.18% and 9.08% for Guangxi,41.08%,16.25% and 8.04% for Guangdong,and 30.78%,15.66% and 7.89% for Yunnan. One of the main active components in Kaempferia galanga Linn. is methoxy cinnamate ethyl cinnamate,which can be inferred that the quality of Kaempferia galanga Linn. from Guangxi and Guangdong is better than that from Yunnan. This experiment also provides evidence for the geoherbalism of Kaempferia galanga Linn.,and provides reference for the further development of the herb.