Simultaneous Determination of Taurine and Aminocaproic Acid in Compound Taurine Eye Drops by High Performance Liquid Chromatography-Refractive Index Detector / 中国药师
China Pharmacist
;
(12): 919-922, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-705628
ABSTRACT
Objective:
To establish an HPLC-RID method for the simultaneous determination of taurine and aminocaproic acid in compound taurine eye drops.Methods:
A Waters NH2column(250 mm ×4.6 mm,5 μm) was used, the mobile phase was water-acetonitrile (4060) at a flow rate of 0.8 ml·min-1, the column temperature was 35.0℃, and the temperatures of the detector reference cell and the sample cell were both set at 35.0℃,The injection volume was 20 μl.Results:
The linear ranges of taurine and aminocaproic acid were 0.05-2.00 mg·ml-1(r = 0.999 9). The average recovery was 98.57% (RSD = 1.07%,n= 9) and 100.69% (RSD =0.75%,n =9),respectively.Conclusion:
The established method is accurate,simple and rapid, and suitable for the quality control of compound taurine eye drops.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2018
Type:
Article
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