Study on HPLC Fingerprints of Olibanum from Different Habitats / 中国药师

ABSTRACT

Objective: To establish the HPLC fingerprint of Olibanum. Methods: The column was Agilent ZORBAX SBC18(250 mm×4. 6 mm,5 μm) with the mobile phase of acetonitrile-0. 1% phosphoric acid (gradient elution) at a flow rate of 1. 0 ml·min-1, the detection wavelength was 270 nm,the column temperature was 30℃,and the injection volume was 10 μl. Olibanum samples from different regions were detected for the characteristic fingerprint. Similarity evaluation software for chromatographic fingerprint of tradi-tional Chinese medicine (2012 edition) was used for the common peaks identification and similarity evaluation. Results: The HPLC fingerprint analysis method for Olibanum was established. The fingerprint consisted of 10 common peaks. The similarities of the finger-prints of twelve samples from different regions were above 0. 95. Conclusion: The method is simple and rapid with good reproducibili-ty, which provides basis for the quality control and evaluation standard of Olibanum.