Simultaneous Determination of Stilbene Glycosides, Ferulic Acid and Paeonol in Qubai Granules by a Dual-wavelength HPLC Method / 中国药师
China Pharmacist
;
(12): 1093-1095, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-705673
ABSTRACT
Objective:
To establish an HPLC determination method for the quantitative indicators in Qubai granules( stilbene gly-cosides, ferulic acid and paeonol).Methods:
A dual-wavelength HPLC assay was used with the following conditions a chromatogra-phy column ODS (150 mm×4. 6 mm, 5 μm) was used, the mobile phase was methanol 0. 1% phosphoric acid with gradient elution, the column temperature was 35℃, and the injection volume was 10 μl.Results:
The linear ranges of the three active constituents were 1.56-156.00 μg·ml-1(r stilbeneglycoside=0.999 8)、1.00~100.00 μg·ml-1(rferulicacid=0.999 8),1.61~161.00 μg·ml-1(rpaeonol=0. 999 7), respectively, and the average recoveries of the three constituents were between 100. 33% and 100. 76% with the RSDS less than 2% (n=6).Conclusion:
The method is simple, stable and reliable, which can be used for the quality control of Qubai granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2018
Type:
Article
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