Simultaneous Determination of Five Active Constituents in Cirsii Herba Dispensing Granules by HPLC-DAD / 中国药师

ABSTRACT

Objective: To develop an HPLC-DAD method for the simultaneous determination of five active constituents (chlorogen-ic acid, baicalin, rutin, linarin and apigenin) in Cirsii Herba dispensing granules. Methods: The chromatographic separation was per-formed on a Thermo Hypersil BDS C18column (250 mm×4. 6 mm,5 μm) with gradient elution by 0. 1% phosphoric acid-acetonitrile as the mobile phase at the flow rate of 1. 0 ml·min-1. The detection wavelength was set at 326 nm,and the column temperature was maintained at 35 ℃. Results: Chlorogenic acid, baicalin, rutin, linarin and apigenin were linear within the range of 0. 072-1. 446 μg (r=0.999 7), 0.043-0.864 μg(r =0.999 5), 0.094 2-1.884 μg (r =0.999 7), 0.150-3.000 μg (r =0.999 8) and 0.043-0. 856 μg (r=0. 999 6), respectively. The recovery was 97. 67% , 98. 19% , 97. 92% ,98. 12% and 98. 02% , and the RSDs was 0. 82% , 0. 92% , 1. 25% , 0. 98% and 1. 17% , respectively. Conclusion: The method is accurate, convenient and reproducible in the quality control of multicomponents in Cirsii Herba dispensing granules.