CEUS diagnosis of acute kidney injury after limb muscle crush injury / 中国医学影像技术

ABSTRACT

Objective To explore the value of quantitative contrast enhanced ultrasonography (CEUS) in early diagnosis and monitoring of acute kidney injury (AKI) after limb muscle crush injury.Methods Totally 64 New Zealand white rabbits were selected and divided into experimental group (n=56) and control group (n=8).Models of AKI were prepared by applying cuff balloon pressure in experimental group,while rabbits in the control group was not given pressure.CEUS of the left kidney was performed at 0.5 h,2.0 h,6.0 h,24.0 h,3 days,7 days,14 days after model establishment.Rabbits in experimental group were divided into different subgroups according to different time points.Peak intensity (PI),area under the curve (AUC),ascending slope (AS) and descending slope (DS) were calculated.The serum levels of creatine kinase (CK),lactic dehydrogenase (LDH),blood urea nitrogen (BUN),creatinine (Cr) were measured.Then the rabbits were sacrificed,and the left renal tissues were fixed for histological observation.Results The levels of CK,LDH,BUN and Cr had statistical differences among all subgroups in experimental group and control group (all P＜0.05),of 2.0,6.0,24.0 h and 3 days subgroups in experimental group were significantly higher than those of control group (all P＜0.05).PI,AUC,AS,DS among all subgroups in experimental group and control group had statistical differences (all P＜0.05).Compared with control group,AS and DS decreased in all subgroups in experimental group (all P＜0.05),but there was no statistical differences among all subgroups in experimental group (P＞0.05).Conclusion CEUS can be used to effectively reflect the changes of AKI after crush injury.