Development and application of a microbiological incubator for medical examination / 中国医学装备

ABSTRACT

Objective: To develop a microbiological incubator used in medical examination and explore its application and effect in bacteriological examination. Methods: 160 microbiological specimens which gathered from clinical examination of hospital were enrolled in research sample group that used the developed microbiological incubator to implement microbial cultivation, while other 160 specimens were enrolled in control group that used an opaque incubator to implement microbial cultivation at the same term. The positive rate of microbiological test, total bacterial count and incubation time of the two groups were compared and analyzed. Results: The positive rates of micrograms in blood, sputum, respiratory tract and urine of the research sample group were 40.00%, 38.71%, 58.82% and 68.42%, respectively, and all of them were significantly higher than that of control group, which positive rates for each item were 13.79%, 19.40%, 35.29% and 22.22%, respectively (x2=10.5247, x2=5.8679, x2=4.0139, x2=7.9435, P<0.05). The total bacterial counts of blood, sputum, respiratory tract and urine of the research sample group were (1.69±0.16)×109/CFU·ml-1, (1.59±0.23)×109/CFU·ml-1, (1.77±0.15)×109/CFU·ml-1and (1.73±0.14)×109/CFU·ml-1, respectively, and all of them were significantly more than that of control group, which total bacterial counts for each item were (1.51±0.26)×109/CFU·ml-1, (1.36±0.14)×109/CFU·ml-1, (1.53±0.27)×109/CFU·ml-1and (1.59±0.11)×109/CFU·ml-1, respectively (t=7.4580, t=10.8049, t=9.8287, t=9.9462, P<0.01). The microbial cultivation time of research sample group that was (2.14±1.20)d was significantly shorter than that of control group that was (3.06±1.14)d (t=7.0308, P<0.01). Conclusion: "The microbiological incubator for medical examination" is used in bacteriological examination can significantly enhance the accuracy of positive detection for microorganism, and significantly increase the number of cultivated bacterial colonies and shorten the cultivation time. Therefore, it has higher value in clinical application and should be popularized.