Study on Content Determination and Pharmacokinetics of Paeoniflorin and Paeonolide from Paeoniae Radix Alba in Rat Plasma / 中国中医药信息杂志

ABSTRACT

Objective To establish a method for the content determination of paeoniflorin and paeonolide in rat plasma by HPLC-MS/MS; To study its characteristics of pharmacokinetics.Methods HPLC-MS/MS method was adopted. The column was Ultimate RXB-C18 (2.1 mm×100 mm, 3 μm) with mobile phase consisted of acetonitrile- 0.1％ formic acid water solution. Flow rate was 0.3 mL/min; column temperature was 30℃; the volume was 5 μL; detection wavelength was 230 nm. Quantitative ions were m/z 525→120.9 (paeoniflorin and paeonolide), m/z 433→224.8 (geniposide). 8 SD male rats were collected blood 0.5 mL from orbital vein 0, 5, 10, 20, 30, 45, 60, 90, 120, 180, 360, 540, 720 min after intragastric administrationBaishaoDecoction 6 g (medicinal materials)/kg to determine the blood concentration of medicine. DAS V2.1.1 software was employed to calculate pharmacokinetic parameters. Results Good linearity was found in the range of 40–8000 ng/mL and 40–10000 ng/mL for paeoniflorin and paeonolide (r=0.9996,r=0.9995). The intra-day and inter-day RSD were less than 6％. The extraction recoveries were 92.33％–95.43％ and 88.65％–95.62％. The matrix effect values were 99.29％–107.86％ and 93.79％–104.05％.All items of method validation were in line with the requirements. The main pharmacokinetic parameters of paeoniflorin and paeonolide were showed as follows Cmax of (8.50±2.01) and (4.13±0.72) μg/mL; tmax of (10.00± 1.73) and (20.00±2.11) min; t1/2 of (142.98±30.11) and (127.68±35.74) min.Conclusion The HPLC-MS/MS method established in this study is simple, feasible, accurate, reliable, highly sensitive, and specific, which can be applied to study the pharmacokinetic characteristics of paeoniflorin and paeonolide in rats in vivo.