Effect of oxymatrine on liver triglyceride metabolism enzymes of apolipoproteinE －/－ mice with fat-induced insulin resistance / 中华老年医学杂志

ABSTRACT

Objective To investigate the effect of oxymatrine on liver triglyceride metabolism enzymes of apolipoprotein E(ApoE)－/－mice with fat-induced insulin resistance. Methods A total of 72 ApoE－/－mice fed with a high-fat diet for 16 weeks were randomly assigned into a model group ,an oxymatrine 25 mg/kg group ,an oxymatrine 50 mg/kg group ,and an oxymatrine 100 mg/kg group , and oxymatrine was administered p.o.for 8 weeks.C57BL/6J mice were selected to serve as the control group.Serum biochemical parameters were assessed ;Insulin resistance was assessed with the Hyper insulinemic-euglycemic clamp test ;Pathological changes in the liver were visualized by hematoxylin ( HE ) staining ;levels of gene expression of triglyceride metabolism enzymes were examined by real-time polymerase chain reaction ( PCR ) and Western-blotting. Results Administration of oxymatrine reduced body weight ,fasting blood glucose , cholesterol ,and triglyceride to varying degrees and alleviated pathological changes in the liver.Glucose infusion rates were(18.5 ± 1.6)mU · kg －1· min－1,(20.1 ± 1.8)mU · kg －1· min－1,and(21.3 ± 2.3)mU · kg －1· min－1in the oxymatrine 25 ,50 ,and 100 mg/kg groups ,respectively ,and were significantly higher than that in the model group (16.5 ± 1.6)mU · kg －1· min－1(all P ＜ 0.05) .mRNA expression levels of hormone-sensitive lipase(HSL) ,adipose triglyceride lipase(ATGL) ,and peroxisome proliferator-activated receptor γ(PPARγ)were higher in the three oxymatrine groups than in the model group ,while levels of diacylglycerol acyltransferase (DGAT1 ) were lower in the oxymatrine groups(F＝53.81 ,21.06 ,23.67 ,35.37 ;all P＜0.05) ;Levels of HSL ,ATGL ,and PPARγ were higher ,while levels of DGAT1 were lower in the oxymatrine 50 and 100 mg·kg －1groups than in the model group ( F ＝ 53.62 ,22.87 ,28.13 ,33.54 ;all P ＜ 0.05 ). Conclusions Oxidative can mitigate insulin resistance in mice fed with a high fat diet through regulating the expression of liver triglyceride metabolism enzymes.