Effect of propofol on invasion of cerebral glioma in rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of propofol on the invasion of cerebral glioma in rats. Methods A total of 120 healthy adult male Wistar rats, weighing 250-280 g, were divided into 4 groups (n=30 each)using a random number table sham operation group(group S), glioma group(group G) and different doses of propofol groups(group P1and group P2). The rats only underwent sphenotresia in group S. The model of brain glioma was established by injecting C6 glioma cells into the right caudate nucle-us in G, P1and P2groups. In P1and P2groups, propofol was infused at the rates of 20 mg·kg-1·h-1 and 40 mg·kg1·h-1, respectively, for 6 h through the tail vein at day 10 after establishing the model. The rats were sacrificed at day 18 after establishing the model, global brains were removed, and glioma was isolated. The weight of glioma was measured. The pathological changes were examined using hematoxylin and eosin staining. Immunohistochemical staining was used to determine the positive expression of glial fi-brillary acidic protein in glioma cells. The expression of vascular endothelial growth factor(VEGF)in both the periphery(within 2 mm diameter)and central region of glioma was detected by Western blot. Results No glioma was found in group S. The marked cavity and necrotic region in the central region of glioma and dense distribution of glioma cells and neovessels in the periphery of glioma were observed, and glial fibril-lary acidic protein was positively expressed in the majority of glioma cells in G, P1and P2groups. Com-pared with group S, the expression of VEGF was significantly up-regulated, and the number of positive cells was increased in G, P1and P2groups(P<0.05). Compared with group G, the weight of glioma was significantly decreased, the expression of VEGF was down-regulated, and the number of positive cells was decreased in P1and P2groups(P<0.05). There were no significant differences in the parameters men-tioned above between group P1and group P2(P>0.05). Conclusion Propofol can inhibit the invasion of cerebral glioma and provides anti-tumor effect in rats.