Effect of gbpapentin on the expression of Nrf2 in dorsal root ganglion neurons of diabetic neuropathic pain rats / 中华内分泌代谢杂志

ABSTRACT

Objective To investigate the effect of systemic administration of gabapentin on the expression of Nrf2 in dorsal root ganglion neurons in a rat model of diabetic neuropathic pain (DNP). Methods 60 male SD rats weighing 200-220 g were randomly divided into 4 groups (n= 15 each) control group (group C), DNP group, saline group (SC + DNP group) and gabapentin group ( GBP + DNP group). Diabetes was induced with streptozocin (70 mg/ kg) subcutaneously. Paw withdrawal mechanical threshold ( PWMT) and paw withdrawal thermal latency (PWTL) were measured at 1d before and 3,7,10,14,21, and 28 d after streptozocin injection, and 21 and 28 d after administration of gabapentin (50 mg·kg-1 ·d-1 ,once) intraperitoneally. Saline and gabapentin (50 mg·kg-1 · d-1 ) were given intraperitoneally once a day for 7 d starting from 15 d after streptozocin injection in saline and gabapentin groups respectively. The expression of Nrf2 was determined at 21 d after streptozocin injection by immune-histochemistry and Western blot. Results Compared with group C, PWMT ( 3. 95 ± 0. 57) was significantly decreased, PWTL(6.3 ± 0.81) was significantly shorten, Nrf2 in expression was significantly decreased in DNP, SC+DNP and group (P<0.05). Compared with SC+DNP group, PWMT(8.2 ± 0.79) was significantly increased, PWTL(9.7 ± 1.13) was significantly prolonged, Nrf2 expression was significantly increased in GBP+DNP group (P<0.05). There was no significant difference in the parameters mentioned above between DNP and SC+DNP group (P>0.05). Conclusion Systemic administration of gabapentin attenuates diabetic neuropathic pain by enhancing the expression of Nrf2 in DRG neurons.