Trametenolic acid B induces the programmed death of human gastric cancer HGC-27 cells / 中成药

ABSTRACT

AIM To study the programmed death effect of trametenolic acid B (TAB) against human gastric cancer HGC-27 cells.METHODS The proliferation of cells was examined by MTT assay;cell apoptosis rate and cell cycle were detected by flow cytometry;cell autophagy was observed by acridine orange staining and transmission electron microscope;expressions of autophagy-related proteins were detected by Western blot.RESULTS 10,20,40 μmol/L Trametenolic acid B had good growth-inhibitory effects on HGC-27 cells,blocked in G0/G1 phase,in a dose-dependent and time-dependent manner,but had no obvious effect on cell apoptosis.After treatment with 20 μmol/L trametenolic acid B for 24 h,a large number of autophagy vacuoles and autophagy bodies were observed under transmission electron microscope,and the proportion of cell autophagy was significantly increased with higher dose of TAB;TAB promoted the transformation of type Ⅰ microtubule-associated protein 1 light chain 3 (LC3 Ⅰ) to type Ⅱ microtubule-associated protein 1 light chain 3 (LC3 Ⅱ),up-regulated the expressions of myosin-like B-cell lymphoma-2 (Bcl-2) interacting protein (Beclin-1) and cyclin-dependent kinase inhibitor 1A (p21),and inhibited the CCND1 gene protein (CyclinD1) expression.CONCLUSION Trametenolic acid B can induce the autophagic death of HGC-27 cells,but it has no significant effect on cell apoptosis.