Simultaneous determination of seven constituents in Supplemented Ermiao Granules by HPLC and establishment of fingerprints / 中成药
Chinese Traditional Patent Medicine
;
(12): 341-346, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-710191
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of phellodendrine chloride,berberine hydrochloride,jatrorrhizine hydrochloride,(R,S)-goitrine,astilbin,hesperidin and atractylenolide Ⅲ in Supplemented Ermiao Granules (Phellodendri chinensis Cortex,Atractylodis Rhizoma,Smilacis glabrae Rhizoma,etc.) and to establish fingerprints.METHODS The analysis of 70% methanol extract of this drug was performed on a 35 ℃ thermostatic Amethyst C18-H column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.05 mol/L potassium dihydrogen phosphate-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,291 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r =1),whose average recoveries were 95.77%-110.36% with the RS-Ds of 0.06%-2.94%.There were twenty common peaks in the fingerprints of fourteen batches of samples with the similarities of more than 0.990,five of which (5-hydroxymethylfurfural,phellodendrine,astilbin,hesperidin and berberine hydrochloride) were identified.CONCLUSION This stable,reliable and reproducible method can be used for the quality control of Supplemented Ermiao Granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2018
Type:
Article
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