Simultaneous determination of seven constituents in Waicha Bailing Tincture by HPLC / 中成药

ABSTRACT

AIM To eslablish an HPLC method for the simultaneous content determination of brazilin,(±) protosappanin B,hydroxysafflor yellow A,safflor yellow A,oxypeucedanin,imperatorin and isoimperatorin in Waicha Bailing Tincture (Angelicae dahuricae Radix,Sappan Lignum,Carthami Flos,etc.).METHODS The analysis of methanol extract of this drug was performed on a Agilent TC-C18 column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 285,403,310 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 5),whose average recoveries were 96.91％-99.59％ with the RSDs of 0.81％-1.19％.CONCLUSION This stable and reliable method can be used for the quality control of Waicha Bailing Tincture.