Decolorization technique optimization for Boletus edulis polysaccharides and the antioxidant activity / 中成药

ABSTRACT

AIM To optimize the decolorization technique for Boletus edulis polysaccharides and to evaluate the antioxidant activity.METHODS With decolorization temperature,resin consumption,polysaccharide concentration and decolorization time as influencing factors,decolorization rate and polysaccharide recovery rate as evaluation indices,orthogonal test was applied to optimizing the decolorization technique on the basis of single factor test.Then the antioxidant activity was determined by Fe2+ chelating power,hydroxyl free radical scavenging activity and reducing power tests.RESULTS The optimal conditions were determined to be 30 ℃ for decolorization temperature,7.5 g/100 mg polysaccharides for S8 resin consumption,1 g/L for polysaccharide concentration,and 2 h for decolorization time,the decolorization rate and polysaccharide recovery rate were 89.34％ and 49.26％,respectively.The polysaccharides demonstrated strong Fe2+ chelating power and hydroxyl free radical scavenging activity with weak Fe3+ reduction power.CONCLUSION This stable and reliable method can be used for the decolorization for Boletus edulis polysaccharides with good antioxidant activity.