Simultaneous determination of six constituents in Maiwei Dihuang Pills by HPLC-DAD / 中成药
Chinese Traditional Patent Medicine
;
(12): 853-857, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-710250
ABSTRACT
AIM To establish an HPLC-DAD method for the simultaneous content determination of six constituents in Maiwei Dihuang Pills (Ophiopogonis Radix,Schisandrae chinensis Fructus,Rehmanniae Radix Praeparata,etc.).METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent ZORBAX SB-C18column (4.6 mm × 150 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.2% phosphate acid) flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,230,236 and 274 nm.RESULTS Deoxyschizandrin,schizandrin B,schisandrin,paeoniflorin,paeonol and loganin showed good linear relationships within the ranges of 10-70,6.5-45.5,33.5-234.5,17-119,31-217 and 34-238 μg/mL (r >0.990 0),whose average recoveries (RSDs) were 99.6% (1.7%),100.4% (1.8%),100.7% (1.8%),102.9% (1.7%),102.2% (1.5%) and 99.7% (1.2%),respectively.CONCLUSION This simple and reproducible method can be used for the rapid quality control of Maiwei Dihuang Pills.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2018
Type:
Article
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