A relevant research of connections between the genetic susceptibility of Parkinson's disease and three single nucleotide polymorphisms in transmembrane protein 175, methylcrotonoyl-coenzyme A carboxylase 1 and alpha-synuclein in northern Chinese Han population / 中华神经科杂志

ABSTRACT

Objective To investigate if there were connections between sporadic Parkinson's disease (PD) and three single nucleotide polymorphisms (SNPs) in transmembrane protein 175 (TMEM175 rs34311866), methylcrotonoyl-coenzyme A carboxylase 1 ( MCCC1 rs12637471 ) and alpha-synuclein (SNCA rs356182) in Northern Chinese Han population , and provide basic data for PD genetic research. Methods The research recruited 310 sporadic PD patients in northern Chinese Han population from the Department of Neurology, the First Hospital of China Medical University between 2008 and 2012, and 339 controls without nervous system manifestations from other departments of the First Hospital of China Medical University during the same period.We applied cleaved amplification polymorphism sequence-tagged sites polymerase chain reaction-restriction fragment length polymorphism method to detect the genotype distributions of the SNPs in the northern Chinese Han population , and calculated relevance with PD of the SNPs by chi-square test.Results According to the data, the allele A of SNCA rs356182 had positive effects on the onset of PD in northern Chinese Han population compared with controls (patient group A%＝20.97%(130/620), control group A% ＝29.20%(198/678), χ2＝11.632, P＝0.001); allele G of MCCC1 rs12637471 (χ2＝0.009, P＝0.926) and allele C of TMEM175 rs34311866 (χ2＝1.369, P＝0.242) showed no significant differences between PD and control groups.Conclusion SNCA rs356182 was related with PD, and TMEM175 rs34311866 (M311Y) as well as MCCC1 rs12637471 showed no correlation with PD in the northern Chinese Han population.