A porcine model of relief of 3 days unilateral ureteral obstruction: study on the damage and self-repairing capability of the kidney / 中华肾脏病杂志

ABSTRACT

Objective To explore the reversibility of early stage tubular interstitial injury as well as the timing of reparation through the pig relief of unilateral ureteral obstruction (R-UUO) model.Methods Eight three-month-old female Guangxi BA-MA mini pigs were selected for the construction of R-UUO models.Five time points were set which were UUO 0 day,UUO 3 days,R-UUO 7 days,R-UUO 14 days,and R-UUO 21 days.Renal function,histological structure,and protein expressions of α-smooth muscle actin (α-SMA),vimentin and E-cadherin were evaluated at different time points.Results After 3 days of UUO,compared with UUO 0 day,serum creatinine levels were increased obviously and the kidney tissues presented varying degrees of damage.The expressions of α-SMA and vimentin were increased and E-cadherin expression was decreased (P ＜ 0.05).Following R-UUO after 3 days of UUO,compared to UUO,serum creatinine levels were significantly decreased.Renal pathological tissue damage was repaired.The expressions of α-SMA and vimentin were decreased and E -cadherin expression was increased (P ＜ 0.05).Conclusions The pig R-UUO animal model may provide a good platform to study the kidney injury and repair.The tubular injury may be fully reversed and repaired when removing the pathogenic factors if the renal tubular injury was at an earlier stage.