Expression of scavenger receptor class type B1 in esophageal squamous cell carcinoma and its effects on cell proliferation and invasion / 中华消化杂志

ABSTRACT

Objective To explore the expression of scavenger receptor class type B 1 (SR-B1) in esophageal squamous cell carcinoma (ESCC) ,and its effects on proliferation and invasion of ESCC cells . Methods From May 2012 to August 2017 ,63 ESCC patients who underwent surgical resection in Henan Provincial People′s Hospital were enrolled and ESCC tissues and corresponding normal tissues were collected . The expression of SR-B1 protein in collected tissues and ESCC cells were detected by immunohistochemistry and Western blotting .ESCC EC1 cells and TE1 cells were transfected with SR-B1 small interfering RNA (siRNA) ,control siRNA ,pcDNA3 .1 and pcDNA3 .1-SR-B1 ,respectively .After transfection ,the expression of SR-B1 protein was examined by Western blotting .The cell proliferation , cell cycle and invasion ability of ESCC EC1 cells and TE1 cells after transfection were determined by cell counting kit-8 (CCK-8) assay ,flow cytometry and Transwell chamber .Chi-square test and t test were performed for statistical analysis .Results The positive rate of SR-B1 protein expression in ESCC tissues was 60 .3％ (38/63) ,which was higher than that of corresponding normal tissues (30 .2％ ,19/63) ,and the difference was statistically significant (χ2=11 .565 , P=0 .001) .The expression of SR-B1 protein in ESCC cells Eca109 ,EC9706 ,EC1 ,TE1 and KYSE70 were 0 .244 ± 0 .012 ,0 .285 ± 0 .018 ,0 .455 ± 0 .016 ,0 .479 ± 0 .019 and 0 .390 ± 0 .022 ,respectively ,which were all higher than that of normal esophageal epithelial cell Het-1A (0 .027 ± 0 .011) ,and the differences were statistically significant (t=23 .252 ,21 .633 ,39 .081 ,36 .010 and 25 .591 ;all P<0 .01) .The expression of SR-B1 protein in ESCC EC1 and TE1 was downregulated by SR-B1 siRNA . The downregulation of SR-B1 protein expression suppressed the proliferation of ESCC EC1 cells and TE1 cells ,increased the percentage of cells at G0/G1 phase of ESCC EC1 cells and TE1 cells ((64 .92 ± 1 .68)％ and (64 .34 ± 0 .94)％ ) ,and reduced the invasion abilities of EC1 and TE1 cells (33 .33 ± 7 .51 and 21 .67 ± 4 .04) .The upregulation of SR-B1 expression promoted the proliferation of ESCC EC1 cells and TE1 cells ,reduced the percentage of cells at G0/G1 phase ((31 .72 ± 1 .30)％ and (33 .12 ± 1 .04)％ ) ,and enhanced cell invasion abilities (285 .33 ± 28 .10 and 247 .33 ± 28 .29) .Conclusions The overexpression of SR-B1 in ESCC may be associated with the occurrence and development of ESCC .Treatment targeting SR-B1 may be a novel therapeutic strategy in ESCC .