The expression of the cytokines TβR1 and part of SMAD family members in bullae of lung and its significance / 中华胸心血管外科杂志

ABSTRACT

Objective This study intended to investigate the expression of TGF-β receptor1 (TβR1),smad2,smad3 and smad4 in bullae of lungs of PSP patients and to examine the role of these cytokines in the pathogenesis of PSP.Methods Bullae of thirty-four PSP patients who received standard video-assisted thoracic surgery were included as study group.Normal lung tissues around the bullae of lung of part of the 34 PSP specimens were used as a self-control.Normal lung tissues from ten patients without pneumothorax associated disease were used as a control.Immunohistochemical (IHC) staining of TβR1,smad2,smad3 and smad4 were performed on the normal lung tissue of control specimens and the bullae of lung of PSP specimens.Immunoreactivity was evaluated based on immunoreactive score(IRS).Statistical analyses were performed using t-test or Fisher exact test.Results (1)Overexpression ofTβR1(P＜0.01),smad2(P=0.023) and smad4(P=0.015) was detected in the bullae of lung of PSP patients compared with normal lung tissue around the bullae of lung.(2) TβR1 (P =0.012),smad2 (P =0.031) and smad4 (P ＜ 0.01) was significantly high-expressed in bullae of lung of PSP patients compared with normal lung tissue of control group.The expression of smad3 in the study group and the control group was not statistically significant(P ＞0.05).However,the absolute value of expression of smad3 in PSP bullae tissue was higher than that in control group(IRS 4.253 ± 1.719 vs.3.260 ± 2.213).Conclusion TβR1,smad2 and smad4 was significantly overexpressed in PSP lesions.These results suggest that an abnormal expression of TβR1,smad2 and smad4 may be involved in the pathogenesis of PSP.