Clinical application of FilmArray respiratory panel in Mycoplasma pneumoniae detection / 中华检验医学杂志

ABSTRACT

Objective To evaluate the clinical efficacy and application value of FilmArray Respiratory Panel(FilmArray RP)for Mycoplasma pneumoniae(MP)detection.Methods Methodological evaluation.A total of 360 nasopharyngeal swabs or sputum samples were collected from hospitalized children with community acquired pneumonia in Shanghai Children′s Medical Center,Shanghai Jiao Tong University School of Medicine(SCMC)from November 2016 to May 2017.Among them, there were 197 males and 163 females.There were 185 cases in 0-2 years old group,116 cases in 3-6 years old group and 59 cases in 7-16 years old group.FilmArray RP and serological test were respectively used to detect MP infection. Polymerase chain reaction(PCR)was also performed on all the positive samples of MP detected by FilmArray RP,negative samples of FilmArray RP but positive by serological test and some negative samples of the two test methods selected by simple random sample.The results of FilmArray RP and serological test,the positive rates of different age groups detected by FilmArray RP and the rates of MP combined with virus were statistically analyzed by χ2test.Results Among 360 patients with community acquired pneumonia,39 (10.83%,39/360)were MP positive detected by FilmArray RP.In contrast,93 patients(25.83%,93/360)were diagnosed as MP infections using serological test.Results from the two methods were statistically significant(χ2=27.05, P=0.00).Among them, 39 cases of MP detected by FilmArray RP were also positive by PCR.Sixty-one negative samples of FilmArray RP but positive by serological test and 22 negative samples of the two test methods selected by simple random sample were also negative by PCR.Among the positive samples detected by FilmArray RP,the positive rate of patients aged 7-16 years(28.81%,17/59) was higher than that of patients aged 0-2 years(5.95%,11/185)and patients aged 3-6 years(9.48%,11/116).The positive rates of different age groups were statistically significant(χ2=24.54, P=0.00).The proportion of MP combined with virus infection in 0-2 years old group(81.82%,9/11)was higher than that in 3-6 years old group(45.45%,5/11)and 7-16 years old group(29.41%,5/17),the difference was statistically significant(χ2=7.41, P=0.03).Conclusions FilmArray RP is a rapid method in MP detection.It has great advantages in the detection of MP and virus mixed infection.