Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis
Annals of Laboratory Medicine
;
: 119-124, 2018.
Article
in English
| WPRIM
| ID: wpr-713436
ABSTRACT
BACKGROUND:
Early detection of tuberculosis (TB) is challenging in resource-poor settings because of limited accessibility to molecular diagnostics. The aim of this study was to evaluate the performance of the loop-mediated isothermal amplification kit (TB-LAMP) for TB diagnosis compared with conventional and molecular tests.METHODS:
A total of 290 consecutive sputum samples were collected from May till September, 2015. All samples were processed using the N-Acetyl-L-cysteine (NALC) NaOH method and tested by smear microscopy, solid and liquid culture, real-time PCR, and TB-LAMP.RESULTS:
The sensitivity of TB-LAMP for smear-positive and smear-negative samples with culture positivity was 92.0% and 58.8%, respectively. TB-LAMP was positive in 14.9% of TB culture-negative samples; however, all those samples were also positive by real-time PCR. In addition, none of the samples positive for nontuberculous mycobacteria by culture were positive by TB-LAMP. The overall agreement between TB-LAMP and real-time PCR was good; however, the concordance rate was significantly lower for real-time PCR positive samples with Ct values of 30–35.CONCLUSIONS:
TB-LAMP could replace smear microscopy and increase TB diagnostic capacity when Xpert MTB/RIF is not feasible because of poor infrastructure.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetylcysteine
/
Sputum
/
Tuberculosis
/
Tuberculosis, Pulmonary
/
Sensitivity and Specificity
/
Diagnosis
/
Pathology, Molecular
/
Real-Time Polymerase Chain Reaction
/
Methods
/
Microscopy
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2018
Type:
Article
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