Your browser doesn't support javascript.
loading
Cryohemolysis, erythrocyte osmotic fragility, and supplementary hematimetric indices in the diagnosis of hereditary spherocytosis
Blood Research ; : 10-17, 2018.
Article in English | WPRIM | ID: wpr-713634
ABSTRACT

BACKGROUND:

Hereditary spherocytosis (HS) is a chronic hemolytic anemia characterized by microspherocytes in the peripheral blood and increased erythrocyte osmotic fragility (EOF). This study evaluated the cryohemolysis test (CHT); initial hemolysis (IH); immediate and incubated hemolysis percentage in 5.5 g/L NaCl (H5.5); mean corpuscular hemoglobin concentration (MCHC); red blood cell distribution width (RDW); and Hb/MCHC, Hb/RDW, and MCHC/RDW ratios for the diagnosis of HS.

METHODS:

Data from 13 patients with HS were evaluated at the Instituto de Bioquímica Aplicada and compared with data from 14 unaffected individuals and 11 patients with anemia due to another etiology. Total blood and reticulocyte counts, CHT, and immediate and incubated EOF were performed in all subjects; sensitivity, specificity, efficiency, and Youden index (YI) were calculated.

RESULTS:

Eight patients with HS had MCHC ≥345 g/L, 10 had RDW ≥14.5%, 12 had IH >5.0 g/L, 11 had immediate H5.5 ≥5%, and 13 had incubated H5.5 ≥50% (the cut-off value to consider HS). The efficiency and YI were immediate H5.5 (0.94–0.85), incubated H5.5 (0.89–0.82), IH (0.89–0.78), MCHC (0.87–0.62), CHT (0.84–0.54), and Hb/MCHC (0.71–0.56), respectively. The calculated ratios could distinguish subjects with HS from unaffected individuals (P 0.05).

CONCLUSION:

Although the CHT and supplementary hematimetric indexes were useful to differentiate individuals with SH from healthy controls, they cannot distinguish from anemias of other etiology. CHT and MCHC, in addition to EOF, are recommended for diagnosing HS patients because of their low cost and efficiency.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osmotic Fragility / Sensitivity and Specificity / Reticulocyte Count / Diagnosis / Erythrocyte Indices / Erythrocytes / Hemolysis / Anemia / Anemia, Hemolytic Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Blood Research Year: 2018 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osmotic Fragility / Sensitivity and Specificity / Reticulocyte Count / Diagnosis / Erythrocyte Indices / Erythrocytes / Hemolysis / Anemia / Anemia, Hemolytic Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Blood Research Year: 2018 Type: Article